Analytical Testing Results for Rosita Wild & Raw EVCLO

A wide range of nutritional tests have been performed on Rosita EVCLO. The “Nutritional Analysis” presented represent only a small fraction of this tests which include vitamins, and specialist chemistry testing.

The Nutrient Results (fat-soluble vitamins, sterols & fatty acids) shown below represent typical values and ranges which have been obtained from analytical testing at the following institutes:

  • NIFES (National Institute of Nutrition and Seafood Research) [20/12/2010]
  • ALS Food & Pharmaceutical (United Kingdom) [10/10/2012]
  • Eurofins Food Testing [19/02/2013]
  • Extra virgin oil was also tested [as far back as: 15.07.2011] by the producer at ECO-Marine using Nofima (the Norwegian Institute of Food, Fisheries and Aquaculture Research) BioLab which specialises in marine raw materials and products. Project no (C3937).
  • Nutrition Analytical Service (NAS), Institute of Aquaculture, University of Stirling, Scotland, United Kingdom [15/11/2013]. The head of NAS, Professor Gordon Bell, has over 30 years of experience in fish nutrition and lipid and nutrition related analysis and has published more than 184 peer-reviewed papers on fish nutrition, fatty acids, lipid soluble nutrients and antioxidants. The Chief Analyst, James Dick, provides over 25 years of experience in lipid methodology and is responsible for Quality Control within the Nutrition Group, the development of chromatographic techniques and is trained in all aspects of complex lipid analysis including HPLC, GC, GC/MS and GC/MS/MS.

Testing

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Nutrient Results

The concentration of fat-soluble nutrients depends on several biological factors, including the size of the fish and the amount of oil contained in the liver. There are also seasonal variations related to the spawning cycle of the fish, climatic conditions, plankton, and other conditions affecting the codfish. Because Rosita EVCLO is a completely unrefined & unprocessed wholefood, the concentration of nutrients will always vary from batch to batch.

The Nutrient Results (fat-soluble vitamins, sterols & fatty acids) shown below represent typical values and ranges which have been obtained from analytical testing at the institutes named above:

 

VITAMIN E (ALPHA-TOCOPHEROL) ANALYSIS
SAMPLE ID mg/teaspoon
Wild & Raw EVCLO 3.7-10
Above values calculated from analysis performed at a number of European testing institutes (named above). Each testing institute analysis has been performed in duplicate.
VITAMIN A (RETINOL) ANALYSIS  
SAMPLE ID iu/teaspoon1
Wild & Raw EVCLO 3900
Above values calculated from analysis performed at a number of European testing institutes (named above). Each testing institute analysis has been performed in duplicate
VITAMIN A2 (3,4-DEHYDRORETINOL) ANALYSIS  
SAMPLE ID mg/kg
Wild & Raw EVCLO > = 0.1
> = Greater Than or Equal To Above values calculated from analysis performed at a number of European testing institutes (named above), and each testing institute analyses has been performed in duplicate.
 
VITAMIN D3 (CHOLECALCIFEROL) ANALYSIS#
SAMPLE ID iu/teaspoon1
Wild & Raw EVCLO 395
Above values calculated from analyses performed at a number of European testing institutes (named above), and each testing institute analyses has been performed in duplicate.
#
The vitamin D in Wild & Raw EVCLO is the same form of vitamin D that yourbody produces when exposed to sunlight and the only form found in significant quantities in unrefined fish liver oils.
 
RATIO VITAMINS A:D3  
SAMPLE ID ratio
Wild & Raw EVCLO Typically less than 10:1
STEROL CONTENT
SAMPLE ID mg/Kg
Wild & Raw EVCLO
CHOLESTEROL 4044-5245
BRASSICASTEROL (PHYTOSTEROL) 40.4
Rest to follow
Above values calculated from analyses performed at a number of European testing institutes (named above), and each testing institute analysis has been performed in duplicate.

 

FATTY ACID CONTENT

Wild & Raw EVCLO contains a wide spectrum of fatty acids including omega 3, 4, 6, 7, 9, 11 etc. The test data for select fatty acids of the total lipid content are shown below. Levels of other fatty acids not shown are available on request.

SAMPLE ID mg/teaspoon
Wild & Raw EVCLO
20:5n-3 (EPA) 510
22:6n-3 (DHA) 700
18:1n-9 (Oleic acid) 1,200
20:1n-9 (Gadoleic acid)* 660
22:1n-11 (Cetoleic acid)* 375
Fatty acids, Sum of Omega 6 Calc. 160
Fatty acids, Sum of Omega 3 Calc. 1,460
Unidentified fatty acids 240
RATIO DHA:EPA
SAMPLE ID ratio
Wild & Raw EVCLO
DHA/EPA 1:1 – 1.3:1
Omega 6/Omega 3 Ratio 0.11

1Vitamin testing is not performed on each batch and may vary from batch to batch, and from season to season. The vitamins are all naturally occurring; nothing is added or destroyed during production.

*Gadoleic acid [which was first noted in cod liver oil] and Cetoleic acid are present in higher amounts in codfish from Northern hemisphere and high latitude waters. These fatty acids are therefore found in higher quantities in genuine cod live oil originating from the Northern hemisphere. Cetoleic acid is also usually present in lower proportions than Gadoleic acid.

This ratio is a very good indicator of genuine Northern hemisphere cod liver oil.

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Nutrition Facts

Wild & Raw Extra-Virgin Cod Liver Oil: 150 ml
Suggested Serving Size: 1 teaspoon (5 ml) daily
Servings per Bottle: Approximately 30

Amount Per Serving

Calories 45    Calories from Fat 45
% Daily Value*
Total Fat 4.5g 7.0%
Saturated Fat 0.9g 4.5%
Trans Fat 0g 0%
Cholesterol 18mg 6%

* Percent Daily Values are based on a caloric intake of 2,000 calories.

Does Not Contain: Gluten, yeast, milk derivatives, synthetic nutrients, artificial colors or flavors.

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Shelf-Life Study Results

[Performed by a third party, independent Norwegian laboratory]

  • Shelf-life of unopened bottles at a temperature of 32° to 39.2°F (0° to 4°C) is 12 months.
  • For storage at -4°F (-20°C) the shelf-life is 2-3 times longer.
  • Shelf-life after the bottles are opened at 32° to 39.2°F (0° to 4°C) is 2-3 months

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Purity

Cod Liver Oil has been tested for purity by leading European institutes and meets all the stringent European requirements.

HEAVY METAL CONTENT [ALS Food & Pharmaceutical (UK): 10/10/2012]
SAMPLE ID ppm
Wild & Raw EVCLO
MERCURY n.d
CADMIUM n.d
LEAD n.d
ARSENIC 1.4*

ppm – parts per million
n.d – not detected
*organic (non-toxic) form

HEAVY METAL ANALYSIS [BioDetection Systems b.v. (BDS): 19/03/2013]
SAMPLE ID ppm
Wild & Raw EVCLO
MERCURY n.d
CADMIUM n.d
LEAD n.d
ARSENIC 1.8*

ppm – parts per million
n.d – not detected (the concentration of the analyte is below the limit of quantification).
*organic (non-toxic) form

HEAVY METAL CONTENT [ALS Food & Pharmaceutical (UK): 10/10/2015]
SAMPLE ID ppm
Wild & Raw EVCLO
MERCURY <0.01 mg / kg
CADMIUM <0.001 mg / kg
LEAD <0.005 mg / kg
ARSENIC 1.417 mg / kg*

ppm – parts per million
n.d – not detected
*mainly organic (non-toxic) form

ORGANIC CONTAMINANTS [Dioxins and PCBs, Benzo(a)pyrene]

Certificate of Analysis from:

  • ALS Food & Pharmaceutical (UK) [10/10/2012]
  • BioDetection Systems b.v. (BDS) [19/03/2013]

 

Cod liver oil has been analyzed in triplicate and each time gives a total WHO-PCDD/F-PCB-TEQ lower than the strict European limits and well within them. The values for the oil are in line with QC parameters.

 

 A. POLYAROMATIC HYDROCARBONS

SAMPLE ID BENZO(A)PYRENE (mg/kg)
Wild & Raw EVCLO <0.001#
#the concentration of the analyte is below the limit of quantification

Above value calculated from analyses performed in duplicate.

 

 B. DIOXINS and PCBs 

  • Sum of dioxins (WHO-PCDD/F-TEQ): 0.20 ng/kg#
  • Sum of dioxins and dioxin-like PCBS (WHO-PCDD/F-PCB-TEQ): 0.899 ng/kg#

#Maximum levels set by the European Commission for thesum of dioxins (WHO-PCDD/F-TEQ) for marine oils (fish body oil, fish liver oil and oils of other marine organisms intended for human consumption): 1.75 ng/kg fat

#Maximum levels set by the European Commission for thesum of dioxins and dioxin like PCBs (WHO-PCDD/F-PCB-TEQ) for marine oils (fish body oil, fish liver oil and oils of other marine organisms intended for human consumption): 6.0 ng/kg fat

 Dioxins (sum of polychlorinated dibenzo-para-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs), expressed as World Health Organisation (WHO) toxic equivalent using the WHO-toxic equivalency factors (WHO-TEFs)) and sum of dioxins and dioxin-like PCBs (sum of PCDDs, PCDFs and polychlorinated biphenyls (PCBs), expressed as WHO toxic equivalent using the WHO-TEFs). WHO-TEFs for human risk assessment based on the conclusions of the World Health Organization (WHO) – International Programme on Chemical Safety (IPCS) expert meeting which was held in Geneva in June 2005 (Martin van den Berg et al., The 2005 World Health Organization Re-evaluation of Human and Mammalian Toxic Equivalency Factors for Dioxins and Dioxin-like Compounds. Toxicological Sciences 93(2), 223–241 (2006))

 

 C. BDS DR CALUX technique: another screening method for dioxins and dioxin-like PCBs

SAMPLE ID Method Parameter Result Unit
Wild & Raw EVCLO DR CALUX PCDD/PCDF and dl-PCBs (total BEQ; semi) compliant (1.7) ng BEQ/kg product

The DR CALUX analysis is done according to p-bds-051.

All DR CALUX analysis results comply with EU requirements as indicated in COMMISSION REGULATION (EC) No 278/2012 (amending Regulation (EC) No 152/2009 as regards the determination of the levels of dioxins and polychlorinated biphenyls; feedstuffs).

 

D. Test Results

WHO
PCDD/F-TEQ
WHO-PCDD/F
PCB-TEQ
PCB
28-52-101-138-153-180
EU REGULATION 1.75 pg/g 6.00 pg/g 200 ng/g
Production date: Apr. 21, 2017 (Testing by EUROFINS Report #440-2017-0424-021 and BDS – Report #11859-1) 0.300 pg/g 1.30 pg/g 83.7 ng/g
Production date: Mar. 27, 2017 (Testing by EUROFINS Report #440-2017-0328-087 and BDS – Report #11724-1) 0.300 pg/g 1.20 pg/g 90.5 ng/g
Production date: Mar. 6, 2017 (Testing by EUROFINS Report #440-2017-0307-065 and BDS – Report #11628-1) 0.300 pg/g 1.00 pg/g 113 ng/g
Production date: Feb. 21, 2017 (Testing by EUROFINS Report #440-2017-0206-058 and BDS – Report #11581-1) 0.460 pg/g 3.50 pg/g 138 ng/g
Production date: Feb. 1, 2017 (Testing by EUROFINS Report ##440-2017-0124-100 and BDS – Report #11433-1) 0.300 pg/g 2.60 pg/g 81.8 ng/g
Production date: Nov. 25, 2016 (Testing by EUROFINS Report ##440-2016-1129-022 and BDS – Report #11170-2) 0.300 pg/g 0.81 pg/g 117.0 ng/g
Production date: Aug 15, 2016 (Testing by EUROFINS Report ##440-2016-0727-020 and BDS – Report #10427-1) 0.300 pg/g 3.80 pg/g 128.0 ng/g
Production date: Jul 25, 2016 (Testing by EUROFINS Report #440-2016-0608-085 and BDS – Report #9786-2) 0.700 pg/g 1.70 pg/g 110.0 ng/g
Production date: May 24, 2016 (Testing by EUROFINS Report #440-2016-0510-063 and BDS – Report #9387-1) 0.700 pg/g 2.00 pg/g 97.9 ng/g
Production date: Apr 04, 2016 (Testing by EUROFINS Report #440-2016-0331-052 and BDS – Report #9005-1) 0.600 pg/g 1.60 pg/g 134 ng/g
Production date: Feb 05, 2016 (Testing by EUROFINS Report #440-2016-0113-005 and BDS – Report #8001-2) 0.400 pg/g 2.70 pg/g 162 ng/g
Production date: Dec 01, 2015 (Testing by EUROFINS – Report #440-2015-1125-102) 0.342 pg/g 3.15 pg/g 178 ng/g
Production date: Oct 15, 2015 (Testing by BDS – Report # 7365-1) 0.110 pg/g 0.70 pg/g 171 ng/g
Production date: Sep 28, 2015 (Testing by EUROFINS – Report #440-2015-0922-007) 0.343 pg/g 3.13 pg/g 172 ng/g

 

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Microbiological Testing

MICROBIOLOGICAL CONTENT [ALS Food & Pharmaceutical (UK): 10/10/2012]
SAMPLE ID cfu/ml
Wild & Raw EVCLO
Total Viable Count <1
Coliforms (presumptive) 0
Escherichia coli (presumptive) <1
Yeasts <2
Moulds <2
Salmonella sp. (ELISA) Not detected / 25g

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Oxidation By-Products

 

TOTOX ANALYSIS
Extra virgin oil was also tested by the producer at ECO-Marine using Nofima (the Norwegian Institute of Food, Fisheries and Aquaculture Research) BioLab which specialises in marine raw materials and products [11/11/2013]. Extra virgin oil was also tested as far back as 15.07.2011
SAMPLE ID PEROXIDE VALUE* ANISIDINE VALUE* TOTOX*
Wild & Raw EVCLO 4.8 2.2 11.8

PV expressed as Meq/Kg
AV expressed as anisidine value units
TOTOX = 2PV+AV

 

TOTOX ANALYSIS
Nutrition Analytical Service, Institute of Aquaculture, University of Stirling, Scotland, United Kingdom [15/11/2013]
SAMPLE ID PEROXIDE VALUE* ANISIDINE VALUE* TOTOX*
Wild & Raw EVCLO 0.38 0 0.76

PV expressed as Meq/Kg
AV expressed as anisidine value units
TOTOX = 2PV+AV

 

TOTOX ANALYSIS
Extra virgin oil was also tested by the producer at ECO-Marine using Nofima (the Norwegian Institute of Food, Fisheries and Aquaculture Research) BioLab which specialises in marine raw materials and products [01/02/2014]. Extra virgin oil was also tested as far back as 15.07.2011
SAMPLE ID PEROXIDE VALUE* ANISIDINE VALUE* TOTOX*
Wild & Raw EVCLO 2.9 6.6 12.4

PV expressed as Meq/Kg
AV expressed as anisidine value units
TOTOX = 2PV+AV

*Peroxide value:
NSF limit = < 10 Meq/Kg
CRN limit = 5 Meq/Kg
GOED limit = 5 Meq/Kg

Stringent European Pharmacopoeia Commision max = 5 Meq/Kg

*Anisidine value:
NSF limit = < 20
CRN limit = 20
GOED limit = 20

Stringent European Pharmacopoeia Commision max = 10

*TOTOX:
NSF limit = < 26 Meq/Kg
CRN limit = 26 Meq/Kg
GOED limit = 26 Meq/Kg

Stringent European Pharmacopoeia Commision max = 20 Meq/Kg

NSF: National Sanitation Foundation International
CRN: Council for Responsible Nutrition
GOED: Global Organization for EPA and DHA Omega-3s

TBAR ANALYSIS Nutrition Analytical Service, Institute of Aquaculture, University of Stirling, Scotland, United Kingdom [15/11/2013]
SAMPLE ID TBARS (mg/kg)
Wild & Raw EVCLO 0.25

Above value calculated from analyses performed in duplicate.

TBARs are expressed as mg/kg for feeds and oil and nmole/g tissue for flesh or other tissues.

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Hydrolytic Rancidity Measures

[Extra virgin oil was also tested by the producer at ECO-Marine using Nofima (the Norwegian Institute of Food, Fisheries and Aquaculture Research) BioLab which specialises in marine raw materials and products: 01/02/2014]

% FREE FATTY ACID ANALYSIS
SAMPLE ID %FFA as Oleic
Wild & Raw EVCLO 0.6
ACID VALUE  
SAMPLE ID mg KOH/g
Wild & Raw EVCLO 1.2

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